Journal: Cell reports

Article Title: Species-specific MARCO-alphavirus interactions dictate chikungunya virus viremia

doi: 10.1016/j.celrep.2023.112418

Figure Lengend Snippet:

Article Snippet: Surface expression of MARCO and mMxra8 was assessed by flow cytometry with monoclonal antibody ED31 (4 μg/mL; Bio-Rad MCA1849; RRID: AB_2140591), polyclonal human MARCO antibody (1 μg/mL; R&D AF7586), or monoclonal murine Mxra8 antibody (4 μg /mL; a gift from M. Diamond, Washington University), followed by secondary antibodies: phycoerythrin (PE)-anti-rat IgG1 antibody (BioLegend 407407; RRID: AB_2565987), fluorescein isothiocyanate (FITC)-anti-goat IgG antibody (Jackson ImmunoResearch 705–096-147; RRID: AB_2340402), and FITC anti-Armenian hamster IgG antibody (BioLegend 405502; RRID: AB_315020), respectively.

Techniques: Control, Virus, Recombinant, Mutagenesis, Transfection, Gene Expression, Clone Assay, Plasmid Preparation, Software, Flow Cytometry

Journal: PLoS ONE

Article Title: Contrasting functional responses of resident Kupffer cells and recruited liver macrophages to irradiation and liver X receptor stimulation

doi: 10.1371/journal.pone.0254886

Figure Lengend Snippet: Irradiation-induced changes in the proportion and functions of resKCs and recMφs. Liver MNCs harvested from normal mice and mice 2 days after irradiation (2 Gy) were analyzed. (A) FACS analysis of F4/80 and CD11b in liver MNCs isolated from control and irradiated mice, with recMφs and resKCs displayed as blue and green dots, respectively. (B) Bar graph of recMφ (blue columns) and resKC (green columns) abundance in control and irradiated mice (n = 13 and 6). (C) Phagocytic activity of recMφs (upper panels) and resKCs (lower panels) against intravenously injected FITC- E . coli in mice with or without irradiation. The percentage of FITC- E . coli -positive cells was calculated relative to non-injected control mice (gray area in each panel). (D) Bar graph of E . coli phagocytosis by recMφs (blue columns) and resKCs (green columns) in control and irradiated mice (n = 5 in each group). (E) Intracellular TNF-α production in recMφs (upper panels) and resKCs (lower panels) stimulated with LPS in vitro . Liver MNCs from control or irradiated mice were stimulated with LPS in vitro for 1.5 h, and intracellular TNF-α was labeled (n = 5 in each group). (F) Bar graph of intracellular TNF-α expression in recMφs (blue columns) and resKCs (green columns) in control and irradiated mice (n = 5 in each group). Data are presented as the mean ± SE (* p <0.05, unpaired Student’s t -test).

Article Snippet: In the case of CLEC4F (C-type lectin domain family 4 F) staining, cells with or without permeabilization of cell membranes were labeled with a polyclonal goat anti-mouse CLEC4F antibody (R&D Systems) and an FITC-donkey polyclonal anti-goat IgG antibody (R&D Systems).

Techniques: Irradiation, Isolation, Control, Activity Assay, Injection, In Vitro, Labeling, Expressing

Journal: PLoS ONE

Article Title: Contrasting functional responses of resident Kupffer cells and recruited liver macrophages to irradiation and liver X receptor stimulation

doi: 10.1371/journal.pone.0254886

Figure Lengend Snippet: (A) The proportions of resKCs and recMφs in the livers of WT, LXRα KO, and LXRα/β KO mice were determined via flow cytometry (n = 4 in each group). (B) Bar graph of recMφ (blue columns) and resKC (green columns) proportions in WT, LXRα KO, and LXRα/β KO mice (n = 4 in each group) Data are presented as the mean ± SE (* p <0.05, one-way ANOVA followed by Tukey’s multiple comparisons). (C) In vivo phagocytic activity of WT mice (n = 6), LXRα KO mice (n = 3), and LXRα/β KO mice (n = 3). Mice were injected with FITC- E . coli , and the phagocytic activity of resKCs (green line) was analyzed 15 min after injection. ResKCs from a mouse without E . coli injection were used as negative controls (gray line). (D) Bar graph of the phagocytotic activity of resKCs (green columns) in WT, LXRα KO, and LXRα/β KO mice (n = 6, 3, 3 in each). Data are presented as the mean ± SE (* p <0.05, one-way ANOVA followed by Tukey’s multiple comparisons) (E, F) WT mice and LXRα/β KO mice were injected with live E . coli , and plasma TNF-α and IL-12 levels were measured at the indicated time points after E . coli injection (n = 3 in each group). Data are presented as the mean ± SE. (* p <0.05, unpaired Student’s t -test).

Article Snippet: In the case of CLEC4F (C-type lectin domain family 4 F) staining, cells with or without permeabilization of cell membranes were labeled with a polyclonal goat anti-mouse CLEC4F antibody (R&D Systems) and an FITC-donkey polyclonal anti-goat IgG antibody (R&D Systems).

Techniques: Flow Cytometry, In Vivo, Activity Assay, Injection, Clinical Proteomics

Journal: Journal of Histochemistry and Cytochemistry

Article Title: Netrin-1-like-immunoreactivity Coexpresses With DCC and Has a Differential Level in the Myenteric Cholinergic and Nitrergic Neurons of the Adult Mouse Colon

doi: 10.1369/0022155418819821

Figure Lengend Snippet: List of Secondary Antibodies Used in Immunofluorescence Assays.

Article Snippet: Donkey antigoat FITC , Merck Millipore , AP180F , 1:300.

Techniques: Immunofluorescence